Molecular method for Bartonella species identification in clinical and environmental samples

J Clin Microbiol. 2008 Feb;46(2):776-9. doi: 10.1128/JCM.01720-07. Epub 2007 Dec 19.

Abstract

A new, efficient molecular method for detection of Bartonella, based on the 16S-23S rRNA intergenic spacer and 16S rRNA amplification by multiplex PCR combined with reverse line blotting, was designed. This assay could simultaneously detect 20 different known species and other Bartonella species not described previously.

Publication types

  • Research Support, Non-U.S. Gov't
  • Validation Study

MeSH terms

  • Bacteriological Techniques / methods*
  • Bartonella / classification*
  • Bartonella / genetics
  • Bartonella / isolation & purification*
  • Bartonella Infections / diagnosis*
  • DNA Primers / genetics
  • DNA, Ribosomal Spacer / chemistry
  • DNA, Ribosomal Spacer / genetics
  • Humans
  • Molecular Sequence Data
  • Nucleic Acid Hybridization / methods*
  • Phylogeny
  • Polymerase Chain Reaction / methods*
  • RNA, Bacterial / genetics
  • RNA, Ribosomal, 16S / genetics
  • RNA, Ribosomal, 23S / genetics
  • Sensitivity and Specificity
  • Sequence Analysis, DNA
  • Sequence Homology, Nucleic Acid

Substances

  • DNA Primers
  • DNA, Ribosomal Spacer
  • RNA, Bacterial
  • RNA, Ribosomal, 16S
  • RNA, Ribosomal, 23S

Associated data

  • GENBANK/EU098127
  • GENBANK/EU098128
  • GENBANK/EU098129
  • GENBANK/EU098130
  • GENBANK/EU098131
  • GENBANK/EU098133
  • GENBANK/EU098134