High-performance liquid chromatography analysis of ten dyes for control of safety of commercial articles

J Chromatogr A. 2008 Feb 1;1179(2):152-60. doi: 10.1016/j.chroma.2007.11.099. Epub 2007 Dec 3.

Abstract

Two high-performance liquid chromatographic methods, with ultraviolet-visible spectrophotometry detection (HPLC-UV/Vis) and with tandem mass spectrometry triple quadrupole interfaced with positive ion mode electrospray ionization detection (HPLC-ESI+-QqQ-MS/MS), for determination and quantification of ten commercial dyes are proposed for control in commercial products. Multiple peaks observed for some of the studied dyes in HPLC-UV/Vis chromatograms forced to obtain structural information by HPLC-ESI-MS/MS method with scan mode. The quality parameters of the two proposed chromatographic methods were evaluated for different requirements of normative, showing detection (LODs) and quantification (LOQs) limits around 60-890 and 200-2990 microg L(-1) for HPLC-UV/Vis, and 4.54-14.3 and 15.0-47.6 microg L(-1) for HPLC-ESI+-QqQ-MS/MS.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromatography, High Pressure Liquid / methods*
  • Coloring Agents / analysis*
  • Coloring Agents / toxicity
  • Manufactured Materials / analysis*
  • Spectrometry, Mass, Electrospray Ionization / methods
  • Spectrophotometry, Ultraviolet
  • Tandem Mass Spectrometry

Substances

  • Coloring Agents