Method to determine enantiomeric excess of glucose by nonchiral high-performance liquid chromatography using circular dichroism detection

Shuang Song; Lu Sun; Lei Yuan; Tiemin Sun; Yun Zhao; Wenjian Zuo; Yue Cong; Xian Li; Jinhui Wang

doi:10.1016/j.chroma.2007.11.092

Method to determine enantiomeric excess of glucose by nonchiral high-performance liquid chromatography using circular dichroism detection

J Chromatogr A. 2008 Feb 1;1179(2):125-30. doi: 10.1016/j.chroma.2007.11.092. Epub 2007 Dec 4.

Authors

Shuang Song¹, Lu Sun, Lei Yuan, Tiemin Sun, Yun Zhao, Wenjian Zuo, Yue Cong, Xian Li, Jinhui Wang

Affiliation

¹ Shenyang Pharmaceutical University, Shenyang 110016, China.

PMID: 18093602
DOI: 10.1016/j.chroma.2007.11.092

Abstract

Using nonchiral high-performance liquid chromatography (HPLC) coupled with a circular dichroism (CD) detector, we developed a new method to determine the enantiomeric excess (e.e.) of glucose derivatized with chromophores (p-bromobenzoate). This method is suitable for microscale analysis of glycoside and saccharide. As an application of this method, the enantiomeric excess of the component glucose of a glucan was assayed.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bromobenzoates / chemistry
Chromatography, High Pressure Liquid / methods*
Circular Dichroism / methods*
Glucans / chemistry
Glucose / analogs & derivatives
Glucose / analysis*
Molecular Conformation
Reproducibility of Results
Stereoisomerism

Substances

Bromobenzoates
Glucans
Glucose