Integrating spatially resolved three-dimensional MALDI IMS with in vivo magnetic resonance imaging

Nat Methods. 2008 Jan;5(1):57-9. doi: 10.1038/nmeth1147. Epub 2007 Dec 16.

Abstract

We have developed a method for integrating three dimensional-volume reconstructions of spatially resolved matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI IMS) ion images of whole mouse heads with high-resolution images from other modalities in an animal-specific manner. This approach enabled us to analyze proteomic profiles from MALDI IMS data with corresponding in vivo data provided by magnetic resonance imaging.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Brain / anatomy & histology*
  • Brain / metabolism*
  • Imaging, Three-Dimensional / methods*
  • Magnetic Resonance Imaging / methods*
  • Mice
  • Peptide Mapping / methods*
  • Proteome / metabolism*
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*
  • Systems Integration
  • Tissue Distribution

Substances

  • Proteome