Abstract
Using an in silico approach, a putative nuclear receptor-interacting protein 0610009K11Rik was identified in mouse testis. We named this gene testis-specific nuclear receptor-interacting protein-1 (Tnrip-1). Tnrip-1 was predominantly expressed in the testis of adult mouse tissues. Expression of Tnrip-1 in the testis was regulated during postnatal development, with robust expression in 14-day-old or older testes. In situ hybridization analyses showed that Tnrip-1 is highly expressed in pachytene spermatocytes and spermatids. Consistent with its mRNA expression, Tnrip-1 protein was detected in adult mouse testes. Immunohistochemical studies showed that Tnrip-1 is a nuclear protein and mainly expressed in pachytene spermatocytes and round spermatids. Moreover, co-immunoprecipitation analyses showed that endogenous Tnrip-1 protein can interact with germ cell nuclear receptor (GCNF) in adult mouse testes. Our results suggest that Tnrip-1 is a testis-specific and GCNF-interacting protein which may be involved in the modulation of GCNF-mediated gene transcription in spermatogenic cells within the testis.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Animals
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Animals, Newborn
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Carrier Proteins / genetics*
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Carrier Proteins / metabolism*
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Cell Nucleus / metabolism*
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DNA-Binding Proteins / metabolism
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Gene Expression Regulation, Developmental
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Male
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Membrane Proteins / genetics*
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Membrane Proteins / metabolism*
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Mice
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Mice, Inbred C57BL
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Nuclear Receptor Subfamily 6, Group A, Member 1
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Organ Specificity
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Pachytene Stage
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Protein Binding
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Receptor-Interacting Protein Serine-Threonine Kinases / genetics*
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Receptor-Interacting Protein Serine-Threonine Kinases / metabolism*
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Receptors, Cytoplasmic and Nuclear / metabolism
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Spermatids / cytology
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Spermatids / metabolism
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Spermatocytes / cytology
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Spermatocytes / metabolism
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Spermatozoa / cytology*
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Spermatozoa / metabolism*
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Testis / cytology
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Testis / metabolism*
Substances
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Carrier Proteins
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DNA-Binding Proteins
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Membrane Proteins
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Nr6a1 protein, mouse
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Nuclear Receptor Subfamily 6, Group A, Member 1
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RNA, Messenger
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Receptors, Cytoplasmic and Nuclear
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Tnrip-1 protein, mouse
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Receptor-Interacting Protein Serine-Threonine Kinases