Synthesis of modified DNA by PCR with alkyne-bearing purines followed by a click reaction

Org Lett. 2008 Jan 17;10(2):249-51. doi: 10.1021/ol7026015. Epub 2007 Dec 13.

Abstract

Alkyne-bearing deazapurine triphosphates were prepared and successfully incorporated into DNA using the polymerase chain reaction (PCR). The obtained alkyne-labeled DNA was successfully used in a click reaction with galactose azide.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Azides / chemistry
  • DNA / chemistry*
  • Galactose / analogs & derivatives
  • Galactose / chemistry
  • Molecular Structure
  • Polymerase Chain Reaction*
  • Purine Nucleotides / chemical synthesis*
  • Purine Nucleotides / chemistry

Substances

  • Azides
  • Purine Nucleotides
  • DNA
  • Galactose