Tandem mass spectrometry in physiology

Physiology (Bethesda). 2007 Dec:22:390-400. doi: 10.1152/physiol.00025.2007.

Abstract

Tandem mass spectrometry coupled to liquid chromatography (LC-MS/MS) allows identification of proteins in a complex mixture without need for protein purification ("shotgun" proteomics). Recent progress in LC-MS/MS-based quantification, phosphoproteomic analysis, and targeted LC-MS/MS using multiple reaction monitoring (MRM) has made LC-MS/MS a powerful tool for the study of cell physiology.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, N.I.H., Intramural
  • Review

MeSH terms

  • Animals
  • Cell Physiological Phenomena*
  • Chromatography, Liquid*
  • Humans
  • Intracellular Signaling Peptides and Proteins / analysis*
  • Phosphoproteins / analysis
  • Proteomics / methods*
  • Signal Transduction*
  • Systems Integration
  • Tandem Mass Spectrometry*

Substances

  • Intracellular Signaling Peptides and Proteins
  • Phosphoproteins