Abstract
Tandem mass spectrometry coupled to liquid chromatography (LC-MS/MS) allows identification of proteins in a complex mixture without need for protein purification ("shotgun" proteomics). Recent progress in LC-MS/MS-based quantification, phosphoproteomic analysis, and targeted LC-MS/MS using multiple reaction monitoring (MRM) has made LC-MS/MS a powerful tool for the study of cell physiology.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, N.I.H., Intramural
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Review
MeSH terms
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Animals
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Cell Physiological Phenomena*
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Chromatography, Liquid*
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Humans
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Intracellular Signaling Peptides and Proteins / analysis*
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Phosphoproteins / analysis
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Proteomics / methods*
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Signal Transduction*
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Systems Integration
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Tandem Mass Spectrometry*
Substances
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Intracellular Signaling Peptides and Proteins
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Phosphoproteins