Reagents for the selective immobilization of oligonucleotides on solid supports

Maksim V Kvach; Igor A Prokhorenko; Alexey V Ustinov; Sergei V Gontarev; Vladimir A Korshun; Vadim V Shmanai

doi:10.1080/15257770701503670

Reagents for the selective immobilization of oligonucleotides on solid supports

Nucleosides Nucleotides Nucleic Acids. 2007;26(6-7):809-13. doi: 10.1080/15257770701503670.

Authors

Maksim V Kvach¹, Igor A Prokhorenko, Alexey V Ustinov, Sergei V Gontarev, Vladimir A Korshun, Vadim V Shmanai

Affiliation

¹ Institute of Physical Organic Chemistry NAS, Minsk, Belarus.

PMID: 18066905
DOI: 10.1080/15257770701503670

Abstract

New reagents (CPGs and phosphoramidites) for automatic solid phase synthesis of modified oligonucleotides were designed. Three oligonucleotides carrying fluorescent label at the 5'-terminus and an anchor group at the 3'-terminus were prepared and their immobilization in orthogonal conditions on solid supports was studied.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Indicators and Reagents / chemical synthesis
Indicators and Reagents / chemistry
Oligonucleotides / chemistry*
Sepharose / chemistry*

Substances

Indicators and Reagents
Oligonucleotides
Sepharose