Matrix-assisted laser desorption/ionization mechanism study with dihydroxybenzoic acid isomers as matrices

Rapid Commun Mass Spectrom. 2008;22(2):130-4. doi: 10.1002/rcm.3343.

Abstract

Desorption and ionization efficiencies of matrix-assisted laser desorption/ionization (MALDI) for various biomolecules with different dihydroxybenzoic acid isomers were studied. No clear relationships were observed between MALDI biomolecule signals vs. gas-phase basicity, proton affinity and ionization potential. This indicates the the gas-phase protonation mechanism is not adequate to explain the observed results.

MeSH terms

  • Hydroxybenzoates / chemistry*
  • Isomerism
  • Oligonucleotides / chemistry
  • Peptides / chemistry
  • Polysaccharides / chemistry
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*

Substances

  • Hydroxybenzoates
  • Oligonucleotides
  • Peptides
  • Polysaccharides