[Identification and expression of non-coding RNAs NC28 and NC119 in human tumors]

Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2007 Oct;29(5):618-21.
[Article in Chinese]

Abstract

Objective: To explore and identify the non-coding RNAs related to tumors.

Methods: We used RT-PCR and Northern blot to analyze non-coding RNAs in tumor tissues and cell lines.

Results: Two predicted non-coding RNAs were confirmed to be expressed in cancer tissues and cell lines by RT-PCR and DNA sequencing. We detected the expression of two non-coding RNA transcripts by Northern blot. The length of NC28 was about 1800 nt, and that of NC119 was about 1200nt.

Conclusions: NC28 and NC119 have a tumor-associated expression pattern. The non-coding RNAs may play a role in the development of tumors.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line, Tumor
  • Humans
  • Neoplasms / metabolism*
  • RNA, Untranslated / biosynthesis*

Substances

  • RNA, Untranslated