Abstract
5'-amino-5'-deoxyguanosine-5'-N-phosphoramidate (GNHP) was synthesized in four steps from guanosine and was found to initiate T7 RNAP-promoted transcriptions to afford 5'-H2N-RNA that can be conjugated to activated esters.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Biotin / chemistry
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Deoxyguanosine / analogs & derivatives*
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Deoxyguanosine / chemistry
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Deoxyguanosine / metabolism
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Molecular Structure
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RNA / chemistry*
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RNA / genetics
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RNA / metabolism*
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Sodium Hydroxide / chemistry
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Solutions
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Transcription, Genetic / genetics
Substances
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5'-amino-5'-deoxyguanosine-5'-N-phosphoramidate
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Solutions
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Sodium Hydroxide
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RNA
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Biotin
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Deoxyguanosine