Synthesis of 5'-amino-5'-deoxyguanosine-5'-N-phosphoramidate and its enzymatic incorporation at the 5'-termini of RNA molecules

Chem Commun (Camb). 2007 Dec 21:(47):5096-8. doi: 10.1039/b712066d. Epub 2007 Oct 11.

Abstract

5'-amino-5'-deoxyguanosine-5'-N-phosphoramidate (GNHP) was synthesized in four steps from guanosine and was found to initiate T7 RNAP-promoted transcriptions to afford 5'-H2N-RNA that can be conjugated to activated esters.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biotin / chemistry
  • Deoxyguanosine / analogs & derivatives*
  • Deoxyguanosine / chemistry
  • Deoxyguanosine / metabolism
  • Molecular Structure
  • RNA / chemistry*
  • RNA / genetics
  • RNA / metabolism*
  • Sodium Hydroxide / chemistry
  • Solutions
  • Transcription, Genetic / genetics

Substances

  • 5'-amino-5'-deoxyguanosine-5'-N-phosphoramidate
  • Solutions
  • Sodium Hydroxide
  • RNA
  • Biotin
  • Deoxyguanosine