Evaluation for protein binding affinity of maghemite and magnetite nanoparticles

J Nanosci Nanotechnol. 2007 Nov;7(11):3706-8.

Abstract

We investigated the protein binding affinity of magnetite (Fe3O4) and maghemite (gamma-Fe2O3) nanoparticles with against non-characterized protein from human lung cancer A549 cell line on sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The binding ability of maghemite was 400 ng/mg. According to the SDS-PAGE results, the protein binding affinity of maghemite nanoparticles is stronger than magnetite nanoparticles. These data suggest that a protein can be detected with maghemite nanoparticles.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line
  • Drug Delivery Systems / methods
  • Ferrosoferric Oxide / chemistry*
  • Ferrous Compounds / chemistry*
  • Humans
  • Lung Neoplasms / chemistry*
  • Materials Testing
  • Nanoparticles / chemistry*
  • Neoplasm Proteins / chemistry*
  • Protein Binding

Substances

  • Ferrous Compounds
  • Neoplasm Proteins
  • ferrous oxide
  • Ferrosoferric Oxide