Background. Autologous chondrocyte transplantation (ACT) is a method in reconstructive surgery. Fetal bovine serum (FBS) is still a "gold standard" for media supplementation in many laboratories. Prion and virus diseases are danger for patients treated witch ACT if FBS is used. The aim of this study was to establish the utility of media for chondrocytes culture supplemented with Autologous Serum (AS) or serum substitute (Ultroser G. UG) instead of FBS.<br /> Material and methods. Primary rabbit articular chondrocytes culture was established and cultures in MHCH (Chondrocytes Culture Medium) medium with 10% FBS during first 3 passages. The influence of different sera in various concentrations of 10%, 5% and 2,5%, while Ultroser G in 2%, 1% and 0,5%. Ceels were transferred in amount of 104 into the 24-well plates containing tested media. After 96 hours cells were counted using trypan blue test and photo documentation was done. Means were compared using Student-T test.<br /> Results. AS in concentration of 10% stimulated cell growth to 14,93+/-2,03 x 104 cell/well. Medium supplemented with the same concentration of FBS allowed to obtain 6,56+/-1,08 x 104 cell/well. Transfer from medium supplemented with 10% of FBS to flask containing Ultroser G stopped proliferation rate. Cell viability in AS and FBS supplemented media was over 90% while 80,8-8809% in UG.<br /> Conclusion. 10% FBS and AS conditioned media are both suitable for chondrocytes culture. AS seems to be the serum conditioner for chondrocytes propagation.