Development of a specific fluorescent probe for 8-oxoguanosine

Nucleic Acids Symp Ser (Oxf). 2007:(51):315-6. doi: 10.1093/nass/nrm158.

Abstract

8-Oxoguanosine (8-oxoG) is derived from the oxidation of guanosine (dG) and is known to induce transversion mutations (G:C to T:A) in DNA. We have already reported the fluorescent probe "8-oxoG-clamp", which shows a selective fluorescence quenching phenomenon toward 8-oxoG. In this study, attachment of an additional fluorophore to 8-oxoG-clamp was investigated as a strategy for the detection of 8-oxoG by a fluorescence color change attributed to the 8-oxoG-clamp.

MeSH terms

  • Base Pairing
  • Carbamates / chemistry*
  • Fluorescent Dyes / chemistry*
  • Guanosine / analogs & derivatives*
  • Guanosine / analysis
  • Pyrimidinones / chemistry*
  • Spectrometry, Fluorescence

Substances

  • Carbamates
  • Cbz-8-oxoG-clamp
  • Fluorescent Dyes
  • Pyrimidinones
  • Guanosine
  • 8-hydroxyguanosine