Targeted gene disruption for the analysis of virulence of Staphylococcus aureus

J Ross Fitzgerald

doi:10.1007/978-1-59745-468-1_8

Targeted gene disruption for the analysis of virulence of Staphylococcus aureus

Methods Mol Biol. 2007:391:103-12. doi: 10.1007/978-1-59745-468-1_8.

Author

J Ross Fitzgerald¹

Affiliation

¹ Centre for Infectious Diseases, Chancellor's Building, New Royal Infirmary, University of Edinburgh, Edinburgh, Scotland, UK.

PMID: 18025672
DOI: 10.1007/978-1-59745-468-1_8

Abstract

In recent years, molecular genetic approaches to the study of the disease pathogenesis of Staphylococcus aureus have resulted in many new biological insights. I describe methods used for targeted disruption of staphylococcal genes leading to loss of gene function, important for studies of staphylococcal proteins and their role in virulence.

MeSH terms

Alleles
Cloning, Molecular
Drug Resistance, Bacterial / genetics
Gene Deletion
Gene Targeting
Genes, Bacterial
Mutagenesis
Plasmids / genetics
Polymerase Chain Reaction / methods
Staphylococcus Phages / genetics
Staphylococcus aureus / drug effects
Staphylococcus aureus / genetics*
Staphylococcus aureus / pathogenicity*
Temperature
Transformation, Genetic
Virulence / genetics