Feasibility of direct oxygenation of primary-cultured rat hepatocytes using polyethylene glycol-decorated liposome-encapsulated hemoglobin (LEH)

Hirosuke Naruto; Hongyun Huang; Masaki Nishikawa; Nobuhiko Kojima; Atsushi Mizuno; Katsuji Ohta; Yasuyuki Sakai

doi:10.1263/jbb.104.343

Feasibility of direct oxygenation of primary-cultured rat hepatocytes using polyethylene glycol-decorated liposome-encapsulated hemoglobin (LEH)

J Biosci Bioeng. 2007 Oct;104(4):343-6. doi: 10.1263/jbb.104.343.

Authors

Hirosuke Naruto¹, Hongyun Huang, Masaki Nishikawa, Nobuhiko Kojima, Atsushi Mizuno, Katsuji Ohta, Yasuyuki Sakai

Affiliation

¹ Institute of Industrial Science (IIS), University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8505, Japan.

PMID: 18023811
DOI: 10.1263/jbb.104.343

Abstract

We tested the short-term efficacy of liposome-encapsulated hemoglobin (LEH) in cultured rat hepatocytes. Supplementation with LEH (20% of the hemoglobin concentration of blood) did not lower albumin production in static culture, and completely reversed the cell death and deterioration in albumin production caused by an oxygen shortage in 2D flat-plate perfusion bioreactors.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Capsules
Cells, Cultured
Coated Materials, Biocompatible / chemistry
Feasibility Studies
Hemoglobins / chemistry*
Hemoglobins / metabolism*
Hepatocytes / drug effects
Hepatocytes / metabolism*
Liposomes / chemistry*
Male
Oxygen / administration & dosage*
Oxygen / metabolism*
Polyethylene Glycols / chemistry*
Rats
Rats, Wistar

Substances

Capsules
Coated Materials, Biocompatible
Hemoglobins
Liposomes
Polyethylene Glycols
Oxygen