Helicobacter pylori (H. pylori) has been found in the upper gastrointestinal tract. It is incriminated as aetiological factor in various pathological conditions. This study was designed to determine whether H. pylori plays a role in the pathogenesis of acute appendicitis. H.pylori was investigated in 40 patients divided in 2 groups. A: patients with abdominal pain and B: patients with acute appendicitis, confirmed by pathology studies. The Ag H.pylori was investigated in the faeces of both groups. In the group B, besides, appendix samples were tested for culture for H. pylori. Confirmation was made by PCR (Polymerase Chain Reaction) analysis. Statistical analysis was made. The positivity for H. pylori infection in the faeces was found in Group A in 15% of the patients and in the group B in 35%. In the group B, besides, the culture of the appendix cecal was positive in 71.4%. Though was observed difference between both groups, this one was not significant. It seems that H. pylori colonizes the appendix in small proportion and is unlikely to be associated in direct correlation with acute appendicitis. However, seropositive patients with acute inflammation are likely to suffer from purulent o gangrenous form.