HPLC analysis of aliphatic sulfonate surfactants using ion-pair detection

Molecules. 2005 Sep 30;10(9):1179-89. doi: 10.3390/10091179.

Abstract

A method was developed for the analysis of a number of surfactants which contained no UV-chromophores, using RP-HPLC with Indirect Photometric Detection, IPD. Pyridinium salts such as N-methylpyridinium iodide, N-methyl-2,2'-dipyridinium iodide and N,N'-dimethyl-2,2'-dipyridinium diiodide, were used as the visualization reagents, forming ion-pair complexes with the sulfonate surfactants. This allowed ordinary UV-detection. N-methylpyridinium iodide proved to be a suitable reagent, both with respect to ease of preparation and response. The eluents consisted of mixtures of acetonitrile and water, being 0.1 - 0.25 mM with respect to pyridinium salt. The method was sensitive and exhibited good signal to noise ratios, as well as linear responses over a wide concentration range. All of the analyzed surfactants were separated, including the diastereomeric forms of some of the surfactants.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alkanesulfonates / analysis*
  • Chromatography, High Pressure Liquid / methods*
  • Photometry
  • Pyridinium Compounds / chemistry
  • Surface-Active Agents / analysis*
  • Time Factors

Substances

  • Alkanesulfonates
  • Pyridinium Compounds
  • Surface-Active Agents
  • 1-methylpyridinium