High-resolution MEA platform for in-vitro electrogenic cell networks imaging

Annu Int Conf IEEE Eng Med Biol Soc. 2007:2007:6086-9. doi: 10.1109/IEMBS.2007.4353737.

Abstract

A platform based on an active-pixel-sensor electrode array (APS-MEA) for high-resolution imaging of in-vitro electrogenic cell cultures is presented, characterized and validated under culture conditions. The system enables full frame acquisition at 8 kHz from 4096 microelectrodes integrated with separations of 21 microm and zoomed area acquisition with temporal resolutions down to 8 micros. This bi-modal acquisition feature opens new perspectives in particular for neuronal activity analysis and for the correlation of micro-scale and macro-scale behaviors. The low-noise performances of the integrated amplifier (11 microVRMS) combined with a hardware implementation reflecting image-/video-concepts enable high-resolution acquisitions with real-time processing capabilities adapted to the handling of the large amount of acquired data.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Action Potentials / physiology*
  • Animals
  • Cell Culture Techniques / instrumentation*
  • Cell Culture Techniques / methods
  • Diagnostic Imaging / instrumentation*
  • Diagnostic Imaging / methods
  • Image Interpretation, Computer-Assisted / instrumentation*
  • Image Interpretation, Computer-Assisted / methods
  • Microelectrodes*
  • Nerve Net / physiology*
  • Rats
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Signal Processing, Computer-Assisted / instrumentation*