There are several methods used for sterilization. In those methods chlorine, heat and UV rays are traditionally used. In recent years, the UV sterilization is taken notice as a sterilization method that the sterilized object does not change in quality and is environment-friendly. In this paper, an UV-LED is focused because it does not contain harmful substance and has longer operating life. The results have showed that complete germicidal effects for E. coli and Vibrio parahaemolyticus by UV-LED exposure of 30 minutes and 10 minutes, respectively. These results suggest that the UV-LED has sterilization effects. Therefore, UV-LED can be used as a sterilization device.