Rapid determination of chloramphenicol residues in aquaculture tissues by immunochromatographic assay

Anal Sci. 2007 Nov;23(11):1281-4. doi: 10.2116/analsci.23.1281.

Abstract

An immunochromatographic assay was developed to detect chloramphenicol (CAP) residues in aquaculture tissues. The limit of detection (LOD) was 10 ng g(-1) for detecting CAP spiked in the aquaculture tissues. The results were confirmed by liquid chromatography tandem mass spectrometry (LC/MS/MS) and indicated that there was a good agreement between the two methods. The linear regression equation was y = 1.19x + 0.539 with R(2) = 0.978. The assay time for test was less than 5 min and the method is suitable for rapid testing on-site.

MeSH terms

  • Animals
  • Anti-Bacterial Agents / analysis
  • Antibodies / immunology
  • Aquaculture*
  • Cells, Cultured
  • Chloramphenicol / analysis*
  • Chromatography / instrumentation*
  • Chromatography / methods*
  • Cross Reactions / immunology
  • Female
  • Fishes*
  • Immunoassay / methods*
  • Linear Models
  • Mice
  • Mice, Inbred BALB C
  • Muscles / chemistry*
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Tandem Mass Spectrometry
  • Time Factors

Substances

  • Anti-Bacterial Agents
  • Antibodies
  • Chloramphenicol