Abstract
We report the synthesis of four oligothiophene-5-labeled deoxyuridines. These modified fluorescent nucleosides have been incorporated into oligodeoxynucleotides designed to be used as probes to discriminate, through changes in fluorescence emission, between hybridization with a perfectly complementary strand and that with a single nucleotide mismatch facing the modified uridines. Upon hybridization, remarkable differences (up to 47%) of the emitted light, depending on the uridine facing base, were observed.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Base Pair Mismatch
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Base Sequence
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Deoxyuridine / analysis
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Deoxyuridine / chemistry*
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Fluorescence
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Fluorescent Dyes / analysis
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Fluorescent Dyes / chemical synthesis
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Fluorescent Dyes / chemistry*
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Genetic Testing*
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Humans
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Oligonucleotides / genetics
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Organophosphorus Compounds / chemistry
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Polymorphism, Single Nucleotide / genetics*
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Spectrometry, Fluorescence
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Thiophenes / chemistry*
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Transition Temperature
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Ultraviolet Rays
Substances
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Fluorescent Dyes
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Oligonucleotides
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Organophosphorus Compounds
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Thiophenes
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phosphoramidite
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Deoxyuridine