Identification of a functional allene oxide synthase-lipoxygenase fusion protein in the soft coral Gersemia fruticosa suggests the generality of this pathway in octocorals

Helike Lõhelaid; Reet Järving; Karin Valmsen; Külliki Varvas; Malle Kreen; Ivar Järving; Nigulas Samel

doi:10.1016/j.bbagen.2007.10.010

Identification of a functional allene oxide synthase-lipoxygenase fusion protein in the soft coral Gersemia fruticosa suggests the generality of this pathway in octocorals

Biochim Biophys Acta. 2008 Feb;1780(2):315-21. doi: 10.1016/j.bbagen.2007.10.010. Epub 2007 Oct 22.

Authors

Helike Lõhelaid¹, Reet Järving, Karin Valmsen, Külliki Varvas, Malle Kreen, Ivar Järving, Nigulas Samel

Affiliation

¹ Department of Chemistry, Tallinn University of Technology, Akadeemia tee 15, 12618 Tallinn, Estonia.

PMID: 17996204
DOI: 10.1016/j.bbagen.2007.10.010

Abstract

The conversion of fatty acid hydroperoxides to allene epoxides is catalysed by a cytochrome P450 in plants. In contrast, in the coral Plexaura homomalla, a catalase-related hemoprotein fused to the lipoxygenase (LOX) was found to function as an allene oxide synthase. This work reports the homology-based RT-PCR cloning and functional expression of a Gersemia fruticosa analogue of the allene oxide synthase-lipoxygenase (AOS-LOX) fusion protein. The G. fruticosa mRNA codes for a protein with 84% sequence identity to the P. homomalla AOS-LOX. Our data indicate that the AOS-LOX fusion protein pathway is used by another coral and P. homomalla represents no exception.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Animals
Anthozoa / enzymology*
Intramolecular Oxidoreductases / chemistry
Intramolecular Oxidoreductases / genetics
Intramolecular Oxidoreductases / metabolism*
Lipoxygenase / chemistry
Lipoxygenase / genetics
Lipoxygenase / metabolism*
Molecular Sequence Data

Substances

Lipoxygenase
Intramolecular Oxidoreductases
hydroperoxide isomerase

Associated data

GENBANK/EU082210