Abstract
It was found that milk of clinical healthy women contains sIgA possessing high affinity for the mammalian thymus DNA and DNA-hydrolyzing activity (sIgA-abzymes). Here we present data that such sIgA-abzymes, purified by sequential chromatography on DEAE-fractogel, heparin-sepharose, DNA-cellulose and followed by gel-filtration, are also able to hydrolyse total RNA from E. coli better than plasmid DNA. Besides, such sIgA-abzymes effectively cleaved 18S and 28S ribosomal RNA isolated from human A549 cells. It is noteworthy that the nuclease activity of sIgA-abzymes was significantly inhibited by ATP, while dATP had no effect on it. A potential role of the ribonuclease activity of sIgA-abzymes present in human milk is discussed.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenosine Triphosphate / metabolism
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Animals
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Antibodies, Catalytic / isolation & purification
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Antibodies, Catalytic / pharmacology*
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Antibody Affinity
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Catalysis
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Cattle
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Chromatography, Ion Exchange
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DNA / metabolism*
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Electrophoresis, Polyacrylamide Gel
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Endonucleases / isolation & purification
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Endonucleases / pharmacology*
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Escherichia coli / metabolism
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Female
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Humans
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Hydrolysis
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Immunoglobulin A, Secretory / isolation & purification
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Immunoglobulin A, Secretory / pharmacology*
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Milk, Human / chemistry*
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Milk, Human / immunology
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RNA, Bacterial / metabolism*
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RNA, Ribosomal / metabolism*
Substances
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Antibodies, Catalytic
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Immunoglobulin A, Secretory
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RNA, Bacterial
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RNA, Ribosomal
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Adenosine Triphosphate
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DNA
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calf thymus DNA
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Endonucleases