Objective: Our goal was to compare the microbial composition of subgingival plaque attached to different types of restorative materials.
Methods: The study included 19 patients. Samples of subgingival plaque were collected using sterile paper points. We cultured on three types of media (tryptic soy agar, non selective Schaedler and selective Schaedler), sample dilution of 1/10(3) and 1/10(4). The identification of anaerobic isolated strains was presumptive.
Results: We isolated an average of 3.22 x 10(5) FCU/ sample of aerobic/facultative bacteria and an average of 6.05 x 10(5) FCU/sample of anaerobic bacteria. Microbial composition was less influenced by the ceramics and artglass.
Conclusions: Changes in microbial composition of subgingival plaque was influenced not only by the material type but also by the technique of restoration. Bacterial retention is enhanced by the wrong placed restoration edges.