Radioiodination of mouse anti-III beta-tubulin antibodies and their evaluation with respect to their use as diagnostic agents for peripheral neuropathies

Appl Radiat Isot. 2008 Mar;66(3):310-6. doi: 10.1016/j.apradiso.2007.09.005. Epub 2007 Sep 19.

Abstract

The monoclonal antibody TU-20 and its scFv fragment were radiolabeled with 125 I in order to develop new imaging agents against the specific neuronal marker III beta-tubulin. The reaction via chloramine-T using thiosulfate as a stopping reductant was determined as the most convenient way for radioiodination. The preserved immunological properties of radioiodinated species were estimated by ELISA, electrophoresis, and immunohistochemistry with autoradiography. Biodistribution studies revealed a different behavior of radioiodinated TU-20 and its scFv.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal / chemistry*
  • Antibodies, Monoclonal / immunology
  • Antibodies, Monoclonal / pharmacokinetics
  • Autoradiography
  • Immunoconjugates / chemistry
  • Immunoconjugates / immunology
  • Immunoconjugates / pharmacokinetics
  • Immunoglobulin Fragments / chemistry
  • Immunoglobulin Fragments / immunology
  • Immunohistochemistry
  • Iodine Radioisotopes / chemistry*
  • Isotope Labeling
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Peripheral Nervous System Diseases / diagnostic imaging*
  • Peripheral Nervous System Diseases / metabolism
  • Radionuclide Imaging
  • Tissue Distribution
  • Tubulin / immunology*

Substances

  • Antibodies, Monoclonal
  • Immunoconjugates
  • Immunoglobulin Fragments
  • Iodine Radioisotopes
  • Tubulin
  • beta3 tubulin, mouse