As an important type of environmental biological resources, macrofungi are vitally useful in our life. Compared with green plants, macrofungi can accumulate high concentrations of heavy metals such as Cd, Pb and Hg. In this paper, the bioaccumulation of heavy metals in macrofungi and the advantages of using macrofungi in ecological remediation of heavy metals pollution were discussed. The main factors affecting the bioaccumulation of heavy metals in macrofungi, include the species, ecological types, bioaccumulation characteristics and genetic potentials of macrofungi, the morphologic traits, parts and lifetime of mycelium and fruiting bodies, the intervals between fructifications, and the ecological environments. It was suggested that to screen out the macrofungi with effective bioaccumulation of heavy metals, be prone to artificially cultured, better adjustable to environments and easily post-disposed would be the important domain to be explored in the future.