Abstract
The synthesis of a Ni-nitrilotriacetic acid (Ni-NTA) attached via a new tyrosine-based linker matrix on monolithic crosslinked poly(vinyl benzyl chloride)/poly(vinylpyrrolidinone) is described. This matrix is incorporated inside a microstructured PASSflow reactor which was used for automatic purification and immobilisation of His(6)-tagged proteins. These could be used as stable and highly active biocatalysts for the synthesis of (R)-benzoin (6), (R)-2-hydroxy-1-phenylpropan-1-one (7) and 6-O-acetyl-D-glucal (17) in a flow-through mode.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Aldehyde-Lyases / isolation & purification*
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Benzoin / chemistry
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Benzoin / metabolism
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Bioreactors*
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Catalysis
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Enzymes, Immobilized / metabolism
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Escherichia coli / enzymology*
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Esters / metabolism
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Hydrolysis
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Lysine / metabolism
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Nickel / metabolism*
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Nitrilotriacetic Acid / metabolism*
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Povidone / metabolism*
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Stereoisomerism
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Tyrosine / metabolism*
Substances
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Enzymes, Immobilized
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Esters
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Tyrosine
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Nickel
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Aldehyde-Lyases
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benzaldehyde lyase
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Povidone
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Lysine
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Nitrilotriacetic Acid
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Benzoin