Characterization of nuclear localization and nuclear export signals of yeast actin-binding protein Pan1

FEBS Lett. 2007 Nov 27;581(28):5371-6. doi: 10.1016/j.febslet.2007.10.036. Epub 2007 Oct 29.

Abstract

Pan1 is an actin patch-associated protein involved in endocytosis. Our studies revealed that in oleate-grown cells Pan1 is located in the nucleus as well as in patches. One of three putative nuclear localization signals (NLS) of Pan1, NLS2, directed beta-galactosidase (beta-gal) to the nucleus. However, GFP-Pan1(886-1219), containing NLS2, was found in the cytoplasm indicating that it may contain a nuclear export signal (NES). A putative Pan1 NES, overlapping with NLS3, re-addressed NLS(H2B)-NES/NLS3-beta-gal from the nucleus to the cytoplasm. Inactivation of the NES allowed NLS3 to be effective. Thus, Pan1 contains functional NLSs and a NES and appears to shuttle in certain circumstances.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Active Transport, Cell Nucleus
  • Cell Nucleus / metabolism*
  • Fungal Proteins / chemistry
  • Fungal Proteins / genetics
  • Fungal Proteins / metabolism*
  • Genes, Reporter / genetics
  • Microfilament Proteins
  • Molecular Sequence Data
  • Nuclear Export Signals*
  • Saccharomyces cerevisiae / chemistry
  • Saccharomyces cerevisiae / cytology
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae / metabolism*
  • Saccharomyces cerevisiae Proteins

Substances

  • Fungal Proteins
  • Microfilament Proteins
  • Nuclear Export Signals
  • PAN1 protein, S cerevisiae
  • Saccharomyces cerevisiae Proteins