Comparison of different DNA extraction and polymerase chain reaction methods to detect Leptospira spp. on field samples

Rev Cubana Med Trop. 2005 Jan-Apr;57(1):51-2.

Abstract

It was carried out a comparison on two reference Leptospira strains DNA between different extraction methods and two polymerase chain reaction protocol. The DNA was quantified and serial dilutions were tested by polymerase chain reaction. The results showed difference in terms of recovery and sensitivity between this methods.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • DNA, Bacterial / analysis
  • DNA, Bacterial / isolation & purification*
  • Italy
  • Leptospira / genetics*
  • Leptospira / isolation & purification
  • Leptospira interrogans serovar pomona / genetics
  • Leptospira interrogans serovar pomona / isolation & purification
  • Leptospirosis / genetics
  • Leptospirosis / veterinary*
  • Polymerase Chain Reaction / methods
  • Polymerase Chain Reaction / veterinary*
  • Sensitivity and Specificity

Substances

  • DNA, Bacterial