Abstract
A multi-serovar ELISA based on the outer membrane Lipoprotein L41 (LipL41) of pathogenic Leptospira was developed to increase sensitivity by using a single test antigen. A sensitivity of 99 % and a specifity of 92 % could be achieved. The established diagnostic polymerase chain reaction is also able to detect fast and reliably pathogenic Leptospira in different clinical samples.
MeSH terms
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Animals
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Enzyme-Linked Immunosorbent Assay / methods
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Enzyme-Linked Immunosorbent Assay / veterinary*
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Gerbillinae
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Leptospira / genetics
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Leptospira / isolation & purification*
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Leptospira / pathogenicity
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Leptospirosis / diagnosis
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Leptospirosis / veterinary*
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Polymerase Chain Reaction / methods
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Polymerase Chain Reaction / veterinary*
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Sensitivity and Specificity
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Swine
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Swine Diseases / diagnosis*