Development of a novel ELISA for serodiagnosis of Leptospirosis and additional detection of pathogenic Leptospira by polymerase chain reaction for veterinary routine diagnostics

Rev Cubana Med Trop. 2005 Jan-Apr;57(1):49-50.

Abstract

A multi-serovar ELISA based on the outer membrane Lipoprotein L41 (LipL41) of pathogenic Leptospira was developed to increase sensitivity by using a single test antigen. A sensitivity of 99 % and a specifity of 92 % could be achieved. The established diagnostic polymerase chain reaction is also able to detect fast and reliably pathogenic Leptospira in different clinical samples.

Publication types

  • Evaluation Study

MeSH terms

  • Animals
  • Enzyme-Linked Immunosorbent Assay / methods
  • Enzyme-Linked Immunosorbent Assay / veterinary*
  • Gerbillinae
  • Leptospira / genetics
  • Leptospira / isolation & purification*
  • Leptospira / pathogenicity
  • Leptospirosis / diagnosis
  • Leptospirosis / veterinary*
  • Polymerase Chain Reaction / methods
  • Polymerase Chain Reaction / veterinary*
  • Sensitivity and Specificity
  • Swine
  • Swine Diseases / diagnosis*