Abstract
Biofilm formation by a strain of Streptococcus suis serotype 2 isolated from a case of meningitis in pigs was characterised. Using a polystyrene microtitre plate assay, S. suis 95-8242 produced a dense biofilm when glucose, fructose or sucrose was used as the carbohydrate source, whereas no biofilm formed in the presence of lactose. Polysaccharide production by the biofilm-forming strain was demonstrated by the Congo red agar assay. Transmission electron microscopy revealed that bacterial cells were surrounded by a thick layer of polycationic ferritin-labelled material. S. suis 95-8242 was more resistant to both penicillin G and ampicillin in biofilms than in planktonic cultures on the basis of minimal inhibitory and minimal bactericidal concentrations.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Anti-Bacterial Agents / therapeutic use*
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Biofilms / growth & development*
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Culture Media / chemistry
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Dose-Response Relationship, Drug
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Drug Resistance, Bacterial
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Kinetics
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Meningitis, Bacterial / drug therapy
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Meningitis, Bacterial / microbiology
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Meningitis, Bacterial / veterinary*
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Microbial Sensitivity Tests / veterinary
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Microscopy, Electron, Transmission / veterinary
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Streptococcal Infections / drug therapy
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Streptococcal Infections / microbiology
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Streptococcal Infections / veterinary*
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Streptococcus suis / drug effects
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Streptococcus suis / physiology*
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Streptococcus suis / ultrastructure
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Swine
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Swine Diseases / drug therapy
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Swine Diseases / microbiology*
Substances
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Anti-Bacterial Agents
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Culture Media