Abstract
Berberine is known to possess a wide variety of pharmacological activities, including pro-apoptotic activity. However, its molecular targets are not elucidated at present. NAG-1 and ATF3 are induced by several dietary compounds associated with pro-apoptotic activity. Berberine induces cell growth arrest, apoptosis, NAG-1, and ATF3 in human colorectal cancer cells. ATF3 induction by berberine is mediated in a p53-dependent manner, whereas NAG-1 induction by berberine is mediated by multiple signaling pathways. Our results suggest that berberine facilitates apoptosis and that NAG-1 and ATF3 expression plays an important role in berberine-induced apoptosis.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Activating Transcription Factor 3 / genetics
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Activating Transcription Factor 3 / metabolism*
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Alkaloids / pharmacology
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Apoptosis / drug effects*
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Berberine / pharmacology*
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Biological Products / pharmacology
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Blotting, Western
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Cell Line, Tumor
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Cell Proliferation / drug effects*
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Colorectal Neoplasms / genetics
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Colorectal Neoplasms / metabolism
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Colorectal Neoplasms / pathology
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Cytokines / genetics
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Cytokines / metabolism*
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Dose-Response Relationship, Drug
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Flow Cytometry
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Growth Differentiation Factor 15
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HCT116 Cells
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Humans
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Isoquinolines / pharmacology
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Luciferases / genetics
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Luciferases / metabolism
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Protein Kinase Inhibitors / pharmacology
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RNA, Small Interfering / genetics
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / metabolism
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Transcription, Genetic / genetics
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Transfection
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Tumor Suppressor Protein p53 / genetics
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Tumor Suppressor Protein p53 / metabolism
Substances
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ATF3 protein, human
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Activating Transcription Factor 3
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Alkaloids
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Biological Products
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Cytokines
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GDF15 protein, human
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Growth Differentiation Factor 15
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Isoquinolines
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Protein Kinase Inhibitors
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RNA, Small Interfering
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Recombinant Fusion Proteins
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Tumor Suppressor Protein p53
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Berberine
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Luciferases
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isoquinoline