Abstract
We analyzed the performance of a single-band Western blot (WB) test using recombinant p26 (rp26) capsid protein of equine infectious anemia virus. According to the results obtained, the rp26 WB test is a reliable confirmatory diagnostic tool to be used as a complementary test after an enzyme-linked immunosorbent assay or agar gel immunodiffusion test yielding doubtful results.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibodies, Viral / analysis*
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Blotting, Western / methods
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Blotting, Western / standards
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Blotting, Western / veterinary*
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Enzyme-Linked Immunosorbent Assay / veterinary
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Escherichia coli / genetics
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Horses
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Immunodiffusion / methods
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Immunodiffusion / standards
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Immunodiffusion / veterinary
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Infectious Anemia Virus, Equine / genetics
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Infectious Anemia Virus, Equine / immunology
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Infectious Anemia Virus, Equine / isolation & purification*
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Recombinant Proteins / chemistry
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Recombinant Proteins / immunology
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Reproducibility of Results
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Serologic Tests / veterinary
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Viral Core Proteins / chemistry*
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Viral Core Proteins / genetics
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Viral Core Proteins / immunology
Substances
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Antibodies, Viral
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Recombinant Proteins
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Viral Core Proteins
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equine infectious anemia virus, p26