Objective: To investigate the expressions of keratinocyte growth factor (KGF), keratinocyte growth factor receptor (KGFR) in CaSki cell and action of keratinocyte growth factor on proliferation, migration of the CaSki cell.
Methods: ELISA and Western blot methods were used to determine the protein expressions of the KGF and KGFR in CaSki cell respectively: 3H-Thymidine incorporation method was used to determine the effect of recombinant human KGF and anti-KGF on CaSki cell proliferation; Millicell-PCF was used to determine the effect of recombinant human KGF, anti-KGF on CaSki cell migration.
Results: Both KGF and KGFR were expressing in the CaSki cell; Recombinant human KGF resulted in a increase in the proliferation and migration of CaSki cells; The proliferation and migration of CaSki cell became weak due to autocrine KGF neutralized by KGF antibodies (P < 0.05).
Conclusion: Current results demonstrate that KGF and KGFR express in CaSki cell; Both autocrine and recombinant human KGF have the effect on CaSki cell proliferation and migration.