Reconstitution of glyphosate resistance from a split 5-enolpyruvyl shikimate-3-phosphate synthase gene in Escherichia coli and transgenic tobacco

Appl Environ Microbiol. 2007 Dec;73(24):7997-8000. doi: 10.1128/AEM.00956-07. Epub 2007 Oct 19.

Abstract

A highly N-phosphonomethylglycine (glyphosate)-resistant Pseudomonas fluorescens G2 5-enolpyruvyl shikimate-3-phosphate synthase (EPSPS) was mapped to identify potential split sites using a transposon-based linker-scanning procedure. Intein-mediated protein complementation was used to reconstitute glyphosate resistance from the genetically divided G2 EPSPS gene in Escherichia coli strain ER2799 and transgenic tobacco.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3-Phosphoshikimate 1-Carboxyvinyltransferase / genetics*
  • 3-Phosphoshikimate 1-Carboxyvinyltransferase / metabolism*
  • Anti-Bacterial Agents / antagonists & inhibitors*
  • Anti-Bacterial Agents / metabolism
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • Escherichia coli / drug effects*
  • Escherichia coli / enzymology
  • Escherichia coli / genetics
  • Escherichia coli / metabolism
  • Glycine / analogs & derivatives*
  • Glycine / antagonists & inhibitors
  • Glycine / metabolism
  • Glyphosate
  • Herbicides / antagonists & inhibitors*
  • Herbicides / metabolism
  • Molecular Sequence Data
  • Nicotiana / drug effects*
  • Nicotiana / enzymology
  • Nicotiana / genetics
  • Nicotiana / metabolism
  • Plants, Genetically Modified / genetics
  • Plants, Genetically Modified / metabolism
  • Pseudomonas fluorescens / enzymology
  • Pseudomonas fluorescens / genetics
  • Sequence Analysis, DNA

Substances

  • Anti-Bacterial Agents
  • DNA, Bacterial
  • Herbicides
  • 3-Phosphoshikimate 1-Carboxyvinyltransferase
  • Glycine

Associated data

  • GENBANK/EF155478