Abstract
A highly N-phosphonomethylglycine (glyphosate)-resistant Pseudomonas fluorescens G2 5-enolpyruvyl shikimate-3-phosphate synthase (EPSPS) was mapped to identify potential split sites using a transposon-based linker-scanning procedure. Intein-mediated protein complementation was used to reconstitute glyphosate resistance from the genetically divided G2 EPSPS gene in Escherichia coli strain ER2799 and transgenic tobacco.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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3-Phosphoshikimate 1-Carboxyvinyltransferase / genetics*
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3-Phosphoshikimate 1-Carboxyvinyltransferase / metabolism*
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Anti-Bacterial Agents / antagonists & inhibitors*
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Anti-Bacterial Agents / metabolism
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DNA, Bacterial / chemistry
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DNA, Bacterial / genetics
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Escherichia coli / drug effects*
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Escherichia coli / enzymology
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Escherichia coli / genetics
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Escherichia coli / metabolism
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Glycine / analogs & derivatives*
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Glycine / antagonists & inhibitors
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Glycine / metabolism
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Glyphosate
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Herbicides / antagonists & inhibitors*
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Herbicides / metabolism
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Molecular Sequence Data
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Nicotiana / drug effects*
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Nicotiana / enzymology
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Nicotiana / genetics
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Nicotiana / metabolism
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Plants, Genetically Modified / genetics
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Plants, Genetically Modified / metabolism
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Pseudomonas fluorescens / enzymology
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Pseudomonas fluorescens / genetics
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Sequence Analysis, DNA
Substances
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Anti-Bacterial Agents
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DNA, Bacterial
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Herbicides
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3-Phosphoshikimate 1-Carboxyvinyltransferase
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Glycine