To date various signal processing techniques have been applied to surface electromyography (SEMG) for feature extraction and signal classification. Compared with traditional analysis methods which have been used in previous application, continuous wavelet transform (CWT) enhances the SEMG features more effectively. This paper presents methods of analysing SEMG signals using CWT and LabVIEW for extracting accurate patterns of the SEMG signals. We used the scalogram and frequency-time based spectrum to plot the power of the wavelet transform and enhance the diagnosis features of the signal. As a result, clinical interpretation of SEMG can be improved by extracting time-based information as well as scales, which can be converted to frequencies. Using the extracted features of the dominant frequencies of the wavelet transform and the related scales, we were able to train and validate an artificial neural network (ANN) for SEMG classification.