Abstract
The coronavirus infectious bronchitis virus (IBV) nucleocapsid (N) protein is an RNA binding protein which is phosphorylated at two conserved clusters. Kinetic analysis of RNA binding indicated that the C-terminal phosphorylation cluster was involved in the recognition of viral RNA from non-viral RNA. The IBV N protein has been found to be essential for the successful recovery of IBV using reverse genetics systems. Rescue experiments indicated that phosphorylated N protein recovered infectious IBV more efficiently when compared to modified N proteins either partially or non-phosphorylated. Our data indicate that the phosphorylated form of the IBV N protein plays a role in virus biology.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Base Sequence
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Binding Sites
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Cell Line
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Coronavirus Nucleocapsid Proteins
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Infectious bronchitis virus / chemistry*
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Infectious bronchitis virus / genetics
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Infectious bronchitis virus / metabolism*
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Kinetics
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Nucleocapsid Proteins / chemistry*
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Nucleocapsid Proteins / genetics
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Nucleocapsid Proteins / metabolism*
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Phosphorylation
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RNA, Viral / genetics
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RNA, Viral / metabolism
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RNA-Binding Proteins / chemistry
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RNA-Binding Proteins / genetics
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RNA-Binding Proteins / metabolism
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Recombinant Proteins / chemistry
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Recombinant Proteins / genetics
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Recombinant Proteins / metabolism
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Spodoptera
Substances
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Coronavirus Nucleocapsid Proteins
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Nucleocapsid Proteins
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RNA, Viral
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RNA-Binding Proteins
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Recombinant Proteins