Extension of a DNA molecule by local heating with a laser

Phys Rev Lett. 2007 Oct 5;99(14):148104. doi: 10.1103/PhysRevLett.99.148104. Epub 2007 Oct 5.

Abstract

Thermal convection and thermophoresis induced by mum-scale local heating are shown to elongate a single DNA molecule. An infrared laser used as a point heat source is converged into a dispersion solution of DNA molecules, which is observed under a fluorescent microscope. The thermal convection around the laser focus manifests as extensional flow for the long DNA chain. A simulation of thermal convection that reproduces the experimental condition provides numerical support for the stretching caused by thermal convection. This DNA elongation technique is a novel method for manipulating the intact single DNA molecules, and it can be applied to a "lab on a chip".

MeSH terms

  • DNA / chemistry*
  • Heating*
  • Lasers*
  • Micromanipulation / methods*

Substances

  • DNA