The effects of platelet-activating factor on the secretion of interleukin-8 and growth-regulated oncogene alpha in human immortalized granulosa cell line (GC1a)

Am J Reprod Immunol. 2007 Nov;58(5):434-9. doi: 10.1111/j.1600-0897.2007.00527.x.

Abstract

Problem: To investigate the role of platelet-activating factor (PAF) in human ovulation, we studied the regulation of interleukin (IL)-8 and growth-regulated oncogene (GRO) alpha in cultured human immortalized granulosa cell line (GC1a).

Method of study: GC1a was cultured in serum-free medium, and incubated with carbamyl-PAF (C-PAF) and/or PAF receptor antagonist (WEB 2086). The supernatants were collected, and IL-8 and GRO alpha were measured by enzyme-linked immunosorbent assay.

Results: After treatment with C-PAF, the levels of IL-8 and GROalpha increased in a time-dependent manner. The levels of IL-8 and GROalpha were significantly increased after treatment with C-PAF in a dose-dependent manner. However, the levels of IL-8 and GROalpha were significantly decreased by treatment with C-PAF and with increasing concentrations of WEB 2086.

Conclusion: Our data indicated that IL-8 and GROalpha were regulated by C-PAF. The results suggested that PAF may play an important role in human pre-ovulatory processes involving IL-8 and GROalpha production.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Azepines / pharmacology
  • Cell Line
  • Chemokine CXCL1 / biosynthesis
  • Chemokine CXCL1 / immunology
  • Chemokine CXCL1 / metabolism*
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Granulosa Cells / drug effects
  • Granulosa Cells / immunology
  • Granulosa Cells / metabolism*
  • Humans
  • Interleukin-8 / biosynthesis
  • Interleukin-8 / immunology
  • Interleukin-8 / metabolism*
  • Ovulation
  • Phospholipid Ethers / pharmacology*
  • Platelet Activating Factor / immunology*
  • Platelet Aggregation Inhibitors / pharmacology
  • Platelet Membrane Glycoproteins / antagonists & inhibitors
  • Platelet Membrane Glycoproteins / immunology
  • Receptors, G-Protein-Coupled / antagonists & inhibitors
  • Receptors, G-Protein-Coupled / immunology
  • Triazoles / pharmacology

Substances

  • Azepines
  • CXCL1 protein, human
  • Chemokine CXCL1
  • Interleukin-8
  • Phospholipid Ethers
  • Platelet Activating Factor
  • Platelet Aggregation Inhibitors
  • Platelet Membrane Glycoproteins
  • Receptors, G-Protein-Coupled
  • Triazoles
  • platelet activating factor receptor
  • WEB 2086
  • 1-O-hexadecyl-2-N-methylcarbamylphosphatidylcholine