Abstract
Problem:
To investigate the role of platelet-activating factor (PAF) in human ovulation, we studied the regulation of interleukin (IL)-8 and growth-regulated oncogene (GRO) alpha in cultured human immortalized granulosa cell line (GC1a).
Method of study:
GC1a was cultured in serum-free medium, and incubated with carbamyl-PAF (C-PAF) and/or PAF receptor antagonist (WEB 2086). The supernatants were collected, and IL-8 and GRO alpha were measured by enzyme-linked immunosorbent assay.
Results:
After treatment with C-PAF, the levels of IL-8 and GROalpha increased in a time-dependent manner. The levels of IL-8 and GROalpha were significantly increased after treatment with C-PAF in a dose-dependent manner. However, the levels of IL-8 and GROalpha were significantly decreased by treatment with C-PAF and with increasing concentrations of WEB 2086.
Conclusion:
Our data indicated that IL-8 and GROalpha were regulated by C-PAF. The results suggested that PAF may play an important role in human pre-ovulatory processes involving IL-8 and GROalpha production.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Azepines / pharmacology
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Cell Line
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Chemokine CXCL1 / biosynthesis
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Chemokine CXCL1 / immunology
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Chemokine CXCL1 / metabolism*
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Enzyme-Linked Immunosorbent Assay
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Female
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Granulosa Cells / drug effects
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Granulosa Cells / immunology
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Granulosa Cells / metabolism*
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Humans
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Interleukin-8 / biosynthesis
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Interleukin-8 / immunology
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Interleukin-8 / metabolism*
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Ovulation
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Phospholipid Ethers / pharmacology*
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Platelet Activating Factor / immunology*
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Platelet Aggregation Inhibitors / pharmacology
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Platelet Membrane Glycoproteins / antagonists & inhibitors
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Platelet Membrane Glycoproteins / immunology
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Receptors, G-Protein-Coupled / antagonists & inhibitors
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Receptors, G-Protein-Coupled / immunology
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Triazoles / pharmacology
Substances
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Azepines
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CXCL1 protein, human
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Chemokine CXCL1
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Interleukin-8
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Phospholipid Ethers
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Platelet Activating Factor
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Platelet Aggregation Inhibitors
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Platelet Membrane Glycoproteins
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Receptors, G-Protein-Coupled
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Triazoles
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platelet activating factor receptor
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WEB 2086
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1-O-hexadecyl-2-N-methylcarbamylphosphatidylcholine