Pyrene-perylene as a FRET pair coupled to the N2'-functionality of 2'-amino-LNA

Dorthe Lindegaard; Andreas S Madsen; Irina V Astakhova; Andrei D Malakhov; B Ravindra Babu; Vladimir A Korshun; Jesper Wengel

doi:10.1016/j.bmc.2007.04.056

Pyrene-perylene as a FRET pair coupled to the N2'-functionality of 2'-amino-LNA

Bioorg Med Chem. 2008 Jan 1;16(1):94-9. doi: 10.1016/j.bmc.2007.04.056. Epub 2007 May 3.

Authors

Dorthe Lindegaard¹, Andreas S Madsen, Irina V Astakhova, Andrei D Malakhov, B Ravindra Babu, Vladimir A Korshun, Jesper Wengel

Affiliation

¹ Nucleic Acid Center, Department of Physics and Chemistry, University of Southern Denmark, Campusvej 55, DK-5230 Odense M, Denmark.

PMID: 17920888
DOI: 10.1016/j.bmc.2007.04.056

Abstract

Detection of nucleic acid hybridization via fluorescence resonance energy transfer (FRET) using pyren-1-ylmethyl and perylen-3-ylmethyl N2'-functionalized 2'-amino-LNA nucleosides incorporated into oligonucleotides exhibited a clear distance dependence of the FRET efficiency, ranging from below 10% when the fluorophores were approximately 40A apart to approximately 90% when the fluorophores were in close proximity.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Fluorescence Resonance Energy Transfer*
Fluorescent Dyes
Nucleic Acid Conformation
Nucleic Acid Hybridization
Oligonucleotide Probes / chemistry*
Perylene / chemistry*
Pyrenes / chemistry*

Substances

Fluorescent Dyes
Oligonucleotide Probes
Pyrenes
Perylene
pyrene