Confirmatory real-time PCR assay for human papillomavirus (HPV) type 52 infection in anogenital specimens screened for HPV infection with the linear array HPV genotyping test

J Clin Microbiol. 2007 Nov;45(11):3821-3. doi: 10.1128/JCM.01145-07. Epub 2007 Sep 26.

Abstract

A novel real-time PCR assay for detection of human papillomavirus type 52 (HPV-52) DNA (RT-52) was evaluated on 265 anogenital samples. RT-52 had a sensitivity of 98.4% and a specificity of 100% compared to conventional HPV-52 typing assays, including hybridization of PGMY products with an HPV-52-specific probe and PCR sequencing of HPV-52 E6.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anal Canal / virology*
  • DNA, Viral / analysis
  • Female
  • Genitalia / virology*
  • Genotype
  • Humans
  • Male
  • Oligonucleotide Array Sequence Analysis
  • Papillomaviridae / genetics
  • Papillomaviridae / isolation & purification*
  • Papillomavirus Infections / diagnosis*
  • Polymerase Chain Reaction / methods*
  • Sensitivity and Specificity

Substances

  • DNA, Viral