1-Phenylethynylpyrene (1-PEPy) as refined excimer forming alternative to pyrene: case of DNA major groove excimer

Irina V Astakhova; Andrei D Malakhov; Irina A Stepanova; Alexey V Ustinov; Stanislav L Bondarev; Alexander S Paramonov; Vladimir A Korshun

doi:10.1021/bc700280h

1-Phenylethynylpyrene (1-PEPy) as refined excimer forming alternative to pyrene: case of DNA major groove excimer

Bioconjug Chem. 2007 Nov-Dec;18(6):1972-80. doi: 10.1021/bc700280h. Epub 2007 Sep 27.

Authors

Irina V Astakhova¹, Andrei D Malakhov, Irina A Stepanova, Alexey V Ustinov, Stanislav L Bondarev, Alexander S Paramonov, Vladimir A Korshun

Affiliation

¹ Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Miklukho-Maklaya 16/10, 117997, Moscow, Russia.

PMID: 17896811
DOI: 10.1021/bc700280h

Abstract

1-Phenylethynylpyrene fluorochrome was studied as meta- and para-derivatives of arabino-uridine-2'-carbamates in ss and dsDNA. 1-PEPy showed red-shifted emission and increased fluorescence quantum yield compared to pyrene. Although 1-PEPy has very short excited lifetime (<2.5 ns), it is able to form inter- and intrastrand excimers on DNA, probably resulting from spatial preorganization of two dye molecules.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Carbohydrates / chemistry
DNA / chemistry*
Fluorescent Dyes / chemistry*
Magnetic Resonance Spectroscopy
Molecular Structure
Morpholines
Nucleic Acid Denaturation
Nucleosides / chemistry
Photochemistry
Pyrenes / chemistry*
Temperature

Substances

1-phenylethynylpyrene
Carbohydrates
Fluorescent Dyes
Morpholines
Nucleosides
Pyrenes
DNA
amorolfine