Development of codominant simple sequence repeat, single nucleotide polymorphism and sequence characterized amplified region markers for the pea root rot pathogen, Aphanomyces euteiches

Hajime O Akamatsu; Niklaus J Grünwald; Martin I Chilvers; Lyndon D Porter; Tobin L Peever

doi:10.1016/j.mimet.2007.08.001

Development of codominant simple sequence repeat, single nucleotide polymorphism and sequence characterized amplified region markers for the pea root rot pathogen, Aphanomyces euteiches

J Microbiol Methods. 2007 Oct;71(1):82-6. doi: 10.1016/j.mimet.2007.08.001. Epub 2007 Aug 9.

Authors

Hajime O Akamatsu¹, Niklaus J Grünwald, Martin I Chilvers, Lyndon D Porter, Tobin L Peever

Affiliation

¹ Department of Plant Pathology, Washington State University, Pullman, WA 99164-6430, USA.

PMID: 17884212
DOI: 10.1016/j.mimet.2007.08.001

Abstract

Three kinds of genetic markers including simple sequence repeats (SSRs), single nucleotide polymorphisms (SNPs) and sequence characterized amplified regions (SCARs) were developed from Aphanomyces euteiches. Of 69 loci tested, seven SSR, two SNP and two SCAR markers were codominantly polymorphic. These codominant markers and dominant markers described herein will facilitate population genetic and evolutionary studies of this important plant pathogen.

MeSH terms

Aphanomyces / classification
Aphanomyces / genetics*
Genetic Markers / genetics*
Pisum sativum / microbiology
Plant Roots / microbiology
Polymorphism, Single Nucleotide
Repetitive Sequences, Nucleic Acid*

Substances

Genetic Markers