Genetic variations in Aeromonas hydrophila isolates from clinical and environmental sources in Thailand

S Korbsrisate; S Trakulsomboon; S Damnin; J Gatedee; O A Ratchtrachenchai; A Leelaporn; B Eampokalap; C Gongviseisoog; S Suparak

Genetic variations in Aeromonas hydrophila isolates from clinical and environmental sources in Thailand

Southeast Asian J Trop Med Public Health. 2007 Jul;38(4):721-7.

Authors

S Korbsrisate¹, S Trakulsomboon, S Damnin, J Gatedee, O A Ratchtrachenchai, A Leelaporn, B Eampokalap, C Gongviseisoog, S Suparak

Affiliation

¹ Department of Immunology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand. grsks@mahidol.ac.th

PMID: 17883013

Abstract

Aeromonas hydrophila, a widely distributed human pathogen causing a variety of diseases, can be isolated from clinical and environmental sources. Analysis in Thailand of 110 isolates of Aeromonas hydrophila by randomly amplified polymorphic DNA-PCR (RAPD-PCR) revealed one specific RAPD pattern group (G) that was associated only with strains from environmental sources. Cytotoxic activity, adhesion to epithelial cells and exoenzyme secretions of A. hydrophila were also investigated. A comparison of isolates with pattern group G with a set of isolates derived from human blood showed low induction of cytotoxicity from those with RAPD pattern group G suggesting low virulence of these strains.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Aeromonas hydrophila / genetics*
Aeromonas hydrophila / isolation & purification*
Aeromonas hydrophila / pathogenicity
DNA, Bacterial / genetics*
Epithelial Cells / microbiology
Genetic Variation / genetics*
Genotype
Hospitals
Humans
Random Amplified Polymorphic DNA Technique*
Ribotyping
Thailand
Virulence Factors

Substances

DNA, Bacterial
Virulence Factors