Characterization of CTX-M-22 and TEM-141 Encoded by a single plasmid from a clinical isolate of Enterobacter cloacae in China

Gang Liu; Bao-Dong Ling; Yong-En Xie; Li Lin; Yun Zeng; Xiang Zhang; Jun Lei

Characterization of CTX-M-22 and TEM-141 Encoded by a single plasmid from a clinical isolate of Enterobacter cloacae in China

Jpn J Infect Dis. 2007 Sep;60(5):295-7.

Authors

Gang Liu¹, Bao-Dong Ling, Yong-En Xie, Li Lin, Yun Zeng, Xiang Zhang, Jun Lei

Affiliation

¹ Pharmaceutical Research Institute and Department of Pharmacology, North Sichuan Medical College, Sichuan, China.

PMID: 17881870

Abstract

We analyzed the resistance to expanded-spectrum cephalosporins of an Enterobacter cloacae clinical isolate, EC002, by transconjugation, isoelectric-focusing analysis, and cloning experiments. It produced two beta-lactamases with isoelectric point values of 5.4 and 8.7, corresponding to TEM-141, a novel variant of TEM-1, and CTX-M-22, encoded by a transferable plasmid.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Base Sequence
Cephalosporins / pharmacology
China
Drug Resistance, Bacterial / genetics
Enterobacter cloacae / enzymology*
Enterobacter cloacae / genetics
Enterobacter cloacae / isolation & purification
Enterobacteriaceae Infections / drug therapy
Enterobacteriaceae Infections / microbiology*
Humans
Klebsiella pneumoniae / genetics
Molecular Sequence Data
Plasmids / genetics*
beta-Lactam Resistance / genetics*
beta-Lactamases / genetics*
beta-Lactamases / metabolism

Substances

Cephalosporins
beta-Lactamases