Laboratory production of 100 base pair DNA molecular weight markers

Microsugar Chang; Jhih-Hao Wang; Han-Jung Lee

doi:10.1016/j.jbbm.2007.08.002

Laboratory production of 100 base pair DNA molecular weight markers

J Biochem Biophys Methods. 2008 Apr 24;70(6):1199-202. doi: 10.1016/j.jbbm.2007.08.002. Epub 2007 Aug 24.

Authors

Microsugar Chang¹, Jhih-Hao Wang, Han-Jung Lee

Affiliation

¹ Department of Life Science, National Dong Hwa University, Hualien 97401, Taiwan.

PMID: 17870177
DOI: 10.1016/j.jbbm.2007.08.002

Abstract

DNA molecular weight markers are routinely used in agarose gel electrophoresis. Here we report a method called PCR-synthesized marker (PSM) to generate DNA molecular rulers by PCR in the laboratory. This strategy can also be used to produce 100 bp RNA molecular weight markers by run-off transcription.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

DNA / chemistry*
DNA / genetics*
Molecular Weight

Substances

DNA