Different porphobilinogen-deaminase forms in wild and mutant strains of Saccharomyces cerevisiae. A possible correlation with its segregants behaviour

Int J Biochem. 1991;23(10):1019-23. doi: 10.1016/0020-711x(91)90139-e.

Abstract

1. Different porphobilinogen-deaminase (PBG-D) enzyme forms were found for D 27 and D 27/C6 (HEM R+) strains of Saccharomyces cerevisiae. 2. PBG-D was partially purified and chromatographed on Sephadex G-100 in either the presence or absence of a protease inhibitor. For D 27 only one active peak was observed while for D 27/C6 strain two active peaks were found. 3. A correlation between this differential behaviour and the presence of HEM R+ gene was looked for employing two segregants of one tetrad from D 27 and D 27/C6 mating.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Ammonia-Lyases / genetics*
  • Ammonia-Lyases / metabolism
  • Chromatography, Gel
  • Isoenzymes / genetics
  • Mutation*
  • Saccharomyces cerevisiae / enzymology*
  • Saccharomyces cerevisiae / genetics

Substances

  • Isoenzymes
  • Ammonia-Lyases
  • porphobilinogenase