Study of caprine beta-casein using reversed-phase high-performance liquid chromatography and mass spectroscopy: identification of a new genetic variant

Golfo Moatsou; Daniel Mollé; Ekaterini Moschopoulou; Gagnaire Valérie

doi:10.1007/s10930-007-9098-8

Study of caprine beta-casein using reversed-phase high-performance liquid chromatography and mass spectroscopy: identification of a new genetic variant

Protein J. 2007 Dec;26(8):562-8. doi: 10.1007/s10930-007-9098-8.

Authors

Golfo Moatsou¹, Daniel Mollé, Ekaterini Moschopoulou, Gagnaire Valérie

Affiliation

¹ Laboratory of Dairy Science and Technology, Department of Food Science and Technology, Agricultural University of Athens, Iera Odos 75, Athens, 118 55, Greece.

PMID: 17846874
DOI: 10.1007/s10930-007-9098-8

Abstract

The genotypes and the main phosphorylation levels of beta-casein of goat milk were studied using RP-HPLC/ESI-MS. A new variant of caprine beta-casein named E has been characterized using RP-HPLC/ESI-MS, MALDI-MS and NanoESI MS/MS methods. Its sequence differed from that of variant A in the mono amino acid substitution D47 --> Y47, which resulted in a 48 Da experimental mass difference between them. The calculated molecular mass of the new variant E 6 P was estimated as 23,869 Da. Its phosphorylation pattern was similar to that of variant A, the most abundant types being those with 5 and 6 P in similar quantities.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Substitution
Animals
Caseins / genetics*
Caseins / isolation & purification
Caseins / metabolism*
Chromatography, High Pressure Liquid / methods*
Genetic Variation*
Goats
Milk / chemistry*
Molecular Sequence Data
Spectrometry, Mass, Electrospray Ionization / methods*
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*

Substances

Caseins