Abstract
The immunological role of the Pasteurella multocida toxin (PMT) in mice was examined using a PMT mutant strain. After a nasal inoculation, the mutant strain failed to induce interstitial pneumonia. Moreover, PMT had no significant effect on the populations of CD4+, CD8+, CD3+, and CD19+ immunocytes in blood or on the populations of CD4+ and CD8+ splenocytes (P<0.01). However, there was a significant increase in the total number of cells in the BAL samples obtained from the wild-type P. multocida-inoculated mice. On the other hand, the level of IL-1 expression decreased when the macrophages from the bronchio-alveolar lavage were stimulated with PMT. Overall, PMT appears to play some role (stimulating and/or inhibiting) in the immunological responses but further studies will be required to confirm this.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antigens, CD19 / immunology
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Bacterial Proteins / immunology*
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Bacterial Toxins / immunology*
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Bronchoalveolar Lavage Fluid / cytology
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Bronchoalveolar Lavage Fluid / immunology
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Bronchoalveolar Lavage Fluid / microbiology
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CD3 Complex / immunology
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CD4-Positive T-Lymphocytes / immunology
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CD8-Positive T-Lymphocytes / immunology
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Cell Count
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Interleukin-1beta / metabolism
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Lung / immunology
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Lung / microbiology
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Lung Diseases, Interstitial / immunology
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Lung Diseases, Interstitial / microbiology
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Mice
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Mice, Inbred BALB C
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Mutation*
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Pasteurella Infections / immunology*
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Pasteurella Infections / microbiology
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Pasteurella multocida / genetics
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Pasteurella multocida / immunology*
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Pasteurella multocida / pathogenicity
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Virulence / genetics
Substances
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Antigens, CD19
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Bacterial Proteins
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Bacterial Toxins
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CD3 Complex
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Interleukin-1beta
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Pasteurella multocida toxin